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. 2021 Mar 24;16(3):e0248355. doi: 10.1371/journal.pone.0248355

Fig 8. MYC-diMF synthetic lethality is not due to AURK inhibition.

Fig 8

(A) Immunofluorescent staining of mitotically arrested RPE-MYC cells for Histone 3 phosphorylated at serine 10 (H3 P-Ser10) as a surrogate for AURKB activity. Staining was at 24 hours of treatment. The AURKB inhibitor AZD1152 (200 nM) was used as a positive control to demonstrate inhibition of phosphorylation. The diMF concentration used (12.5 μM) was previously found to induce maximum cell death and polyploidy. (B) Immunofluorescent staining of RPE-MYC cells for the AURKA activating phosphorylation mark at Threonine 288 (AURKA P-Thr288). Staining was at 24 hours of treatment. The AURKA inhibitor MLN8237 (100 nM) inhibits accumulation of AURKA phosphorylated at Thr288 at the centrosomes of spindle poles. diMF was used at 12.5 μM. DAPI was used as a DNA stain in both (A) and (B).