Fig. 1. Growth factor sequestration with varying modified simulated body fluid (mSBF) treatments. (A) Schematic of experiment. Hydrated scaffolds and scaffolds soaked in mSBF for 1, 3, or 7 days were used to sequester BMP2. (B) Sequestration of BMP2 after varying treatment times in mSBF. Mineralized collagen scaffolds are capable of sequestering BMP2 without mSBF treatments. Groups that share a letter are not significantly different (p < 0.05). (C) Retention of BMP2 within scaffolds for 7 days. Mineralized collagen scaffolds had the highest retention compared to all mSBF treated groups by day 7. Groups that share a letter within a day are not significantly different (p < 0.05). * indicates significant differences between all groups within a day. Note: the y-axis starts at 90% to better show differences between groups. To see plots with the y-axis 0–100% please see ESI Fig. 1.† DATA: boxplots overlaid with individual data points are used to represent data.