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. 2020 Nov 9;3(4):227–236. doi: 10.1093/abt/tbaa022

Figure 1.

Figure 1

Characterization of IBI104 by antigen binding/blocking. (A) Binding affinity of IBI104 to soluble human-TIM-3 by SPR. IBI104 was used as 2 ug/mL, Tim-3 antigen was diluted for six 2-fold dilutions from 100 nM. KD, binding dissociation equilibrium constant; Ka, kinetic association rate; Kd, kinetic dissociation rate. (B) Binding affinity of IBI104 to soluble Cyno-TIM-3 by BLI. IBI104 was used as 2 ug/mL, Tim-3 antigen was diluted for six 2-fold dilutions from 100 nM. KD, binding dissociation equilibrium constant; Ka, kinetic association rate; Kd, kinetic dissociation rate. (C, D) Graphs showing cell binding of IBI104 to Human-TIM-3-expressing CHO-S and cyno-TIM-3 expressing CHO-S. IgG1 is the isotype control. (E) Binding of pre-activated T cells. (F) The PtdSer-blocking ability of IBI104 was analyzed in L363 cells. Apoptosis was induced in L363 by using H2O2. All experiments were repeated in at least two independent sets showing representative results.