Fig 4. Target biomass composition and comparison between simulated and experimental biomass elemental ratios for the transition of iTps1432 from exponential to stationary phase under nitrogen-limited conditions.

(a) Biomass composition was measured 0, 1, 3, 7, and 10 days after transfer to fresh media with low nitrate and used to calculate the biomass objective function at each time point. (b) C:N, N:P, and C:P ratios were measured 0, 1, 2, 3, 5, 7, and 10 days after transfer and compared with simulated biomass elemental composition. See S4 Fig for plots of error rates between predictions and experimental measurements.