Fig 1. Generation Meflin-CreER^T2 mice.

a) First, BAC carrying the Meflin gene was introduced together with a zeocin resistance gene (rZeocin) downstream of the translation start point Met. Next, counter-selection using a CreER^T2 fragment with the 5’ and 3’ homologous arms of the Meflin gene was performed. b) Southern blot analysis. Genomic DNA was digested with Sph1 and detected using a Meflin-ORF probe. The transgenic allele was identified at 4.9 kb, and the wild-type allele was identified at 2.9 kb.