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. 2021 Mar 24;7(13):eabe5043. doi: 10.1126/sciadv.abe5043

Fig. 6. Simultaneous interaction with ITGB1 and PSMA4 is necessary for circPABPC1 function.

Fig. 6

(A) circPABPC1 mutations that selectively block its binding with ITGB1 (left) or PSMA4 (right). (B) Wild-type circPABPC1 and mutants expressed in HA22T cells were isolated by RNA pull-down, and copurified ITGB1 and PSMA4 were probed. (C) Endogenous ITGB1 level and FAK activation in HCC cells expressing wild-type or mutant circPABPC1 were determined by Western blot. (D) circPABPC1 expression in HCC cells (C) was measured by RT-qPCR. (E) HA22T cells expressing the indicated circPABPC1 variants were examined for adhesion to the ECM substrates. OD540nm of crystal violet-stained cells was measured (means ± SD, *P < 0.05, **P < 0.01, and ***P < 0.001, one-way ANOVA). (F) Trypsin digestion assay of HCC cells expressing the indicated circPABPC1 variants.