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. 2021 Apr 1;12(2):386–403. doi: 10.14336/AD.2020.0812

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copyright: © 2021 Yu et al.

this is an open access article distributed under the terms of the creative commons attribution license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 9. — Analyses of free fatty acids and identification of candidate biomarkers. A) Comparison of free fatty acid levels detected by untargeted lipidomics in the HC, VS and MCS groups. Data represent as the mean ± SEM; *P<0.05, ***P<0.001, one-way ANOVA. B) Experimental flow for obtaining the results from targeted lipidomics. C-F) Difference analysis for free fatty acids detected by targeted lipidomics between the VS and MCS groups and the HC group. Data represent as the mean ± SEM; *P<0.05, **P<0.01, ****P<0.0001, n.s.: no significant difference, one-way ANOVA. DHA: docosahexaenoic acid; AA: arachidonic acid. G) Decision tree for predicting differences in certain lipid species and their levels between VS and MCS. H) ROC curves for PC(38:5)-H and AA for use in distinguishing VS patients from MCS patients. AUC, area under the receiver-operating characteristic curve. I) Level of PC(38:5)-H and AA correlated to CRS-R scores. CRS-R: Coma Recovery Scale-Revised; HC: healthy controls; VS: vegetative state; MCS: minimally conscious state.