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. Author manuscript; available in PMC: 2021 Apr 26.
Published in final edited form as: Nat Chem Biol. 2020 Oct 26;17(2):205–212. doi: 10.1038/s41589-020-00669-3

Extended Data Fig. 8. Characterization of pathway intermediates from overexpression of cytOsCPS4 + cytOsKSL4 + CYP99A3 + CYP76M8 + OsMAS + CYP76M14 + CYP701A8 via MEV engineering.

Extended Data Fig. 8

GC-MS total ion chromatogram (TIC) and MS spectra of products from overexpression of HMGR, cytGGPPS, cytOsCPS4, cytOsKSL4, CYP99A3, CYP76M8, OsMAS, CYP76M14, and CYP701A8 in N. benthamiana. Representative TICs are shown for the experimental sample and control. (a) Two products with m/z corresponding to diterpene derived scaffolds were observed only upon expression of pathway genes. The products identified corresponded to momilactone B and 7. Putative ion structures were assigned as reference. Structures of 7 is preliminary and based on a predicted chemical formula combined with analysis of the fragment ions observed in the MS spectra. (b) The major product identified corresponded to momilactone B (See Supplementary Figure 11 and 12 for structural characterization).