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. Author manuscript; available in PMC: 2021 Apr 26.
Published in final edited form as: Nat Chem Biol. 2020 Oct 26;17(2):205–212. doi: 10.1038/s41589-020-00669-3

Extended Data Fig. 4. Characterization of pathway intermediates from overexpression of cytOsCPS4 + cytOsKSL4 + CYP99A3 + CYP76M8 via MEV engineering.

Extended Data Fig. 4

GC-MS total ion chromatogram (TIC) and MS spectra of products from overexpression of HMGR, cytGGPPS, cytOsCPS4, cytOsKSL4, CYP99A3, and CYP76M8 in N. benthamiana. Representative TICs are shown for the experimental sample and controls. Products with m/z corresponding to diterpene derived scaffolds were observed only upon expression of pathway genes. Putative ion structures for 4, 5, and 2G were assigned as reference. Putative structures are preliminary and based on predicted formulas combined with analysis of the fragment ions observed in the MS spectra.