Fig. 1.

Workflow and summary of identified and quantified proteins by region. a, Experimental workflow used in this study. Samples for each region were randomized into two batches such that each batch contained one pool (QC) and at least one individual from each of the four sample groups. The colored tubes indicate the different TMT¹¹-plex reagents. b, Overlap of identified proteins across the three brain regions. c, Overlap of quantified proteins across the three brain regions. Quantified proteins were identified in both batches with TMT quantification data for ≥ 80% of TMT channels across batches. Brain image modified from “Human brain on white background” by _DJ_ used under CC BY-SA 2.0. Abbreviations: TMT, tandem mass tags; IPL, inferior parietal lobule; GP, globus pallidus; CN, cognitively normal; AD, Alzheimer’s disease; QC, quality control.