Table 2. Oligos and sgRNAs.
| Oligo | Sequence | Use |
|---|---|---|
| oMS266 | /5InvddT/G*G*G*A*A*CCTCTTCTGTAACTCCTTAGC | Amplify HDR template |
| oMS267 | /5InvddT/C*C*T*G*A*G*GGCAAACAAGTGAGCAGG | Amplify HDR template |
| oMS269 | TCGTGCCAGCCCCCTAATCT | Validate eIF2Bα tagging |
| oMS270 | CTGAACGGCGCTGCTGTAGC | Validate eIF2Bα tagging |
| oMS256 | AGTGAACTCTACCATCCTGA | Validate eIF2Bβ tagging |
| oMS258 | TTAGGTGGACTCCTGTGC | Validate eIF2Bβ tagging |
| oMS096 | CTGGCTAACTGGCAGAACC | Validate eIF2Bδ tagging |
| oMS268 | AGAAACAAAGGCAGCAGAGT | Validate eIF2Bδ tagging |
| sgMS001 | CAATCTGCTTAGGACACGTG | Target Cas9 to eIF2BβC-terminus |
| sgMS004 | AGAGCAGTGACCAGTGACGG | Target Cas9 to eIF2Bδ C-terminus |
| sgMS006 | GAGGACGCCATGGACGACAA | Target Cas9 to eIF2Bα N-terminus |
| HDR: homology-directed recombination. | ||