Table 3. Antibodies for western blotting.
| Antibody target | Host | Dilution | Manufacturer | Blocking conditions |
|---|---|---|---|---|
| GAPDH | Rabbit | 1/2000 | Abcam | TBS-T + 3% BSA |
| eIF2Bα | Rabbit | 1/1000 | ProteinTech | TBS-T + 3% milk |
| eIF2Bβ | Rabbit | 1/1000 | ProteinTech | TBS-T + 3% milk |
| eIF2Bδ | Rabbit | 1/1000 | ProteinTech | TBS-T + 3% milk |
| eIF2Bε | Mouse | 1/1000 | Santa Cruz Biotechnology | PBS-T + 3% milk |
| ATF4 | Rabbit | 1/1000 | Cell Signaling | PBS-T + 3% milk |
| eIF2α-P | Rabbit | 1/1000 | Cell Signaling | PBS-T + 1% BSA |
| eIF2α | Rabbit | 1/1000 | Cell Signaling | PBS-T + 3% milk |
| eIF2β | Rabbit | 1/1000 | ProteinTech | PBS-T + 3% milk |
| BSA: bovine serum albumin. | ||||