Table 2. SV-AUC properties of Arc207 and Nuc147 molecules.
Despite the higher mass of the Arc207 complex, it sediments slower than the Nuc147 system due to increased particle elongation (frictional ratio, ‘f/fo’). ‘MWthe’ denotes the theoretical molecular weight, and ‘MWGA-MC’ corresponds to the molecular weight derived via GA-MC analysis. Parenthetical values outline the 95% confidence interval of each GA-MC value.
| Nuc147 | Arc207 | 207 DNA | |
|---|---|---|---|
| MWthe (kDa) | 200.3 | 231.4 | 127.8 |
| MWGA-MC (kDa) | 218.6 (207.2, 229.9) |
266.5 (256.9, 276.1) |
156.6 (152.4, 160.1) |
| f/fo | 1.55 (1.49, 1.61) |
1.94 (1.89, 1.99) |
3.14 (3.08, 3.20) |
| Sedimentation Coefficient (S) | 11.2 (11.1, 11.2) |
10.6 (10.6, 10.7) |
6.1 (6.1, 6.1) |