Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | Adipoq-Cre | Jackson Laboratory | RRID:IMSR_JAX:028020 | |
| Strain, strain background (Mus musculus) | Adipoq-rtTA | PMID:22451920 | RRID:IMSR_JAX:033448 | |
| Strain, strain background (Mus musculus) | TRE-Cre | Jackson Laboratory | RRID:IMSR_JAX:006234 | |
| Strain, strain background (Mus musculus) | Prdm16lox/lox | PMID:24439384 | RRID:IMSR_JAX:024992 | |
| Antibody | Anti-tyrosine hydroxylase (Rabbit polyclonal) | Millipore | Cat# AB152, RRID:AB_390204 | IF(1:200) |
| Antibody | Anti-tyrosine hydroxylase (Sheep polyclonal) | Millipore | Cat# AB1542, RRID:AB_90755 | IF(1:200) |
| Antibody | Anti-CD31/PECAM-1 (Goat polyclonal) | R and D Systems | Cat# AF3628, RRID:AB_2161028 | IF(1:200) |
| Antibody | Anti-UCP1 (Rabbit polyclonal) | Abcam | Cat# ab10983, RRID:AB_2241462 | IF(1:200) |
| Antibody | Anti-LYVE1 (Rabbit polyclonal) | Abcam | Cat# ab14917, RRID:AB_301509 | IF(1:200) |
| Antibody | Anti-PRDM16 (Sheep polyclonal) | R and D Systems | Cat# AF6295, RRID:AB_10717965 | WB(1:500) |
| Antibody | Anti-Lamin A/C (Mouse monoclonal) | Santa Cruz Biotechnology | Cat# sc-376248, RRID:AB_10991536 | WB(1:2000) |
| Antibody | Anti-Rabbit IgG (H+L), Alexa Fluor 568 (Donkey polyclonal) | Thermo Fisher Scientific | Cat# A10042, RRID:AB_2534017 | IF(1:200) |
| Antibody | Anti-Rabbit IgG (H+L), Alexa Fluor 647 (Donkey polyclonal) | Thermo Fisher Scientific | Cat# A32795, RRID:AB_2762835 | IF(1:200) |
| Antibody | Anti-Sheep IgG (H+L), Alexa Fluor 647 (Donkey polyclonal) | Thermo Fisher Scientific | Cat# A-21448, RRID:AB_2535865 | IF(1:200) |
| Antibody | Anti-Goat IgG (H+L), Alexa Fluor 568 (Donkey polyclonal) | Thermo Fisher Scientific | Cat# A-11057, RRID:AB_2534104 | IF(1:200) |
| Software, algorithm | Imaris | Bitplane | http://www.bitplane.com/imaris/imaris; RRID:SCR_007370 | |
| Software, algorithm | FilamentTracer | Bitplane | http://www.bitplane.com/imaris/filamenttracer; RRID:SCR_007366 |