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Validation of mRNA expression genes in the PI3K-AKT signaling pathway using qRT-PCR on the same sample set of the microarray study.
| Gene | Fold changea (microarray) | Fold change (qRT-PCR) | Fold change (qRT-PCR replication) |
|---|---|---|---|
| ITGA2 | 17.05 | 30.12 | 26.59 |
| FGF1 | 2.69 | 3.24 | 5.36 |
| COL2A1 | 2.15 | 2.97 | 3.51 |
| BCL2L11 | 3.04 | 2.31 | 2.83 |
| MYC | -3.35 | -3.59 | -3.81 |
| IL7R | -3.29 | -4.29 | -2.73 |
aFold change represents the difference multiple of gene expression between the tension group and the control group.
