Figure 4 |. Superposition of the structures of A2058-methylated 70S ribosome and unmethylated 70S ribosome-ERY complex.

(a) Chemical structure of classical macrolide antibiotic erythromycin (ERY). The standard numbering of carbon atoms is shown in grey. (b) Superposition of the current structure of A2058-methylated 70S ribosome with the new high-resolution structure of ribosome-bound ERY. Methylated A2058 residue is shown in blue with the methyl groups highlighted in orange. Unmethylated A2058 residue is shown in light blue. All structures were aligned based on domain V of the 23S rRNA. (c, d) Close-up views of the desosamine 2´-OH group (c) and the desosamine dimethyl-amino moiety (d) relative to the methyl groups on m⁶₂A2058 residue. Note that there are no sterical clashes between the methyl groups of m⁶₂A2058 and the desosamine 2´-OH group (c) or the desosamine dimethyl-amino moiety (d) of ERY.