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. Author manuscript; available in PMC: 2021 Jul 18.
Published in final edited form as: Nat Chem Biol. 2021 Jan 18;17(4):394–402. doi: 10.1038/s41589-020-00710-5

Extended Data Fig. 2. Doubling times in E. coli of constructs expressing SSAPs and SSBs reveal that co-expression of SSB can dramatically influence toxicity.

Extended Data Fig. 2

(a) Growth curve of cognate SSAP-SSB pairs and SSAPs alone in E. coli under constant induction (7hrs). (b) Doubling time measurements for all combinations of the 4 SSAPs and SSBs in E. coli under constant induction (7hrs) with mean and standard deviation presented for at least 3 biologically independent replicates. The SSAPs vary in toxicity, with λ-Red β showing considerable toxicity. The co-expression of SSBs reduces SSAP toxicity in a number of cases, especially for PaSSB. There are a number of constructs with low toxicity and high genome editing efficiency (λ-Red β + EcSSB, λ-Red β + PaSSB, PapRecT + PaSSB) showing that there is no direct correlation between toxicity and genome editing efficiency.