Extended Data Figure 5. Evaluating FKBP5 effect on T cell activation.

a, Top 5 variable importance in prediction (VIP) genes by partial least squares discrimination analysis (PLS-DA) of RNA-seq data from three caloric-load conditions. b, Representative protein blots and quantitative changes normalized by Actin of canonical TFs of Th1 (TBX21), Th2 (GATA3), and Th17 (RORC) in FKBP5-overexpression (OE) in CD4⁺ T cells isolated from healthy volunteers, 3 days following TCR activation. Bar graph represent mean±SEM with data point of each health volunteer (n=8 subjects, Wilcoxon two-sided, paired analysis). c-d, CD4⁺ T cells were isolated from healthy volunteers and transfected with FKBP5 siRNA and scrambled controls. c, Relative FKBP5 RNA expression in CD4⁺ T cells (Bar graph represent mean±SEM with normalized value to scrambled control, n=6 subjects, values represent average of quadruplicates, two-sided, paired Student’s t-test). d, Cytokine release of IFNγ, IL-4 and IL-17 following FKBP5 knockdown (KD) in CD4⁺ T cells isolated from healthy volunteers, 3 days following TCR activation. The dot and line graphs represent mean value of each subject (n=6 biologically independent subjects, values represent average of duplicates, two-sided, ratio paired Student’s t-tests).

Source Data Extended Data Fig. 5: Unprocessed immunoblots.