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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: J Neurosci Methods. 2021 Feb 4;353:109093. doi: 10.1016/j.jneumeth.2021.109093

Figure 1: Identification and sampling of motor neurons.

Figure 1:

A shows specific labeling of phrenic motor neurons via retrograde labeling with Alexa 488-conjugated CTB is readily apparent in z-projection images of horizontal sections (100 μm thick) of cervical (~C4-C5 pictured) spinal cord. The blue squares show every 20th phrenic motor neuron, which was then assessed for mitochondrial volume density. B shows a survey section (~10 μm thick) containing a putative phrenic motor neuron (inside blue square). These putative phrenic motor neurons later underwent serial block face EM. Scale Bars: A=200 μm; B=20 μm.