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The top row shows a portion with an absence of labeled phrenic motor neurons (left), albeit with a reliable MitoTracker Red fluorescent signal. The bottom row shows little non-specific fluorescence on the 575-630 nm channel when an Alexa 488-conjugated labeled phrenic motor neuron was imaged without any intrathecal application of MitoTraker Red (i.e., a negative control). Scale Bar: 5 μm.
