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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: J Neurosci Methods. 2021 Feb 4;353:109093. doi: 10.1016/j.jneumeth.2021.109093

Figure 2: Confocal imaging.

Figure 2:

A and B show an individual z-slice of CTB and MitoTracker red (MitoR) fluorescence, respectively. C shows the sequential imaging of CTB labeled phrenic motor neurons and the MitoTracker red signal, with a z-step of 0.5 μm. Cyan pseudo-colored examples of the binarisation (D) and surface creation (E) of an individual phrenic motor neuron soma are illustrated. Scale Bars: A=10 μm, B=10 μm, C=5 μm, D=8 μm, E=3 μm.