Skip to main content
. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: J Neurosci Methods. 2021 Feb 4;353:109093. doi: 10.1016/j.jneumeth.2021.109093

Figure 4: Thresholding of MitoTracker Red.

Figure 4:

A shows a 3D z-projection of deconvolved MitoTracker Red signal within a phrenic motor neuron. B Under-thresholding of deconvolved MitoTracker Red signal, with an abundance of signal uncaptured by the binarisation (fuchsia). C shows an acceptable thresholding of deconvolved MitoTracker Red signal, with an abundance of discrete elements and no intense fluorescence outside the binarised volumes. D shows an overly inclusive thresholding of deconvolved MitoTracker Red signal, where excessive enlargement of volumes and many superfused elements becoming indistinguishable from one another. Scale Bar: 3 μm.