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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: J Neurosci Methods. 2021 Feb 4;353:109093. doi: 10.1016/j.jneumeth.2021.109093

Figure 6: Serial blockface EM.

Figure 6:

Serial blockface EM z-stacks (containing ~80-250 z-slices per motor neuron; in this example 162 slices) were assessed using the Cavalieri sampling technique. Within the bounds of the putative phrenic motor neuron (blue outline) mitochondrial areas were manually traced, with their area, circularity, perimeters, long- and short-axes recorded. At 2 μm intervals from the center mid-nuclear motor neuron section, this process was repeated in both directions. The mean area density of mitochondria were taken for each Cavalieri z-slice, and a mitochondrial volume density calculated based on the number of total slices assessed and the distance between each slice (2 μm). Scale Bars: B=5 μm.