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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: J Neurosci Methods. 2021 Feb 4;353:109093. doi: 10.1016/j.jneumeth.2021.109093

Figure 8: Mitochondrial volume density.

Figure 8:

A Silhouette of mitochondrial elements based on deconvolved and binarised MitoTracker Red fluorescence within an identified phrenic motor neuron (dashed blue line). B Silhouette of mitochondria traced over serial blockface EM samples within a putative phrenic motor neuron (dashed blue line). C Scatter-plot (median ± 95% CI) showing no difference in mitochondrial volume density estimates between MitoTracker Red (red circles, n=125) and serial blockface EM (blue squares, n=10) techniques (P=0.91; Mann Whitney U-test). Scale Bar: 5 μm.