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DFs and MSCs exhibit closer perivascular association than do LFs in SC-EC co-cultures. Alpha smooth muscle actin (αSMA) expression on (A) day 7 and (B) day 14. Representative immunofluorescence staining for αSMA (green) counterstained with UEA (red) and DAPI (blue) to show the position of SCs relative to vessels. All samples are from condition 2. White arrowheads depict the sheath-like association of αSMA+ SCs to UEA+ microvessels.
