Table 2.
QTL for grain yield and thousand grain weight in Excalibur/Kukri DH population
| Chr | QTL | Flanking markers | QTL peak | Term | P value | QTL effect |
|---|---|---|---|---|---|---|
| Grain yield | kg/ha | |||||
| 1A | QYld.aww-1A | X3021844–gwm0558 | 45.2–46.8 | Main effect | < 0.001 | 15.3 |
| 1B | QYld.aww-1B.1 | X1134677–X1054439 | 60.2–66.1 | Q * E | < 0.01 | − 274 to 173 |
| 1B | QYld.aww-1B.2 | X2263055–X1061145 | 84.2–84.9 | Main effect | < 0.01 | 14.5 |
| 1B | QYld.aww-1B.3 | Xwmc0830–Xpsp3100 | 105.3–110.5 | Q * E | < 0.01 | − 240 to 127 |
| 1D | QYld.aww-1D | X2243386–1126683 | 70.3–84.1 | Q * E | < 0.01 | − 305 to − 113 |
| 2A | QYld.aww-2A.1 | X1128252–X985175 | 0–2.4 | Main effect | < 0.01 | − 11.1 |
| 2A | QYld.aww-2A.2 | X1051900–X1072243 | 71.8–72.5 | Q * E | < 0.001 | − 79 to 395 |
| 2A | QYld.aww-2A.3 | Xwmc0602–X1131447 | 103.6–105.7 | Q * E | < 0.01 | − 61 to 121 |
| 2B | QYld.aww-2B.1 | X3064619–X100005743 | 9.1–16.4 | Q * E | < 0.0001 | − 175 to 200 |
| 2B | QYld.aww-2B.2 | X1075921–X3021359 | 52.2–52.9 | Main effect | < 0.01 | 11.3 |
| 2B | QYld.aww-2B.3 | X1102573–X1010970 | 70.4–71 | Q * E | < 0.0001 | 37–238 |
| 2D | QYld.aww-2D.1 | X2242328–X991014 | 19.4–28.2 | Q * E | < 0.001 | 38–323 |
| 2D | QYld.aww-2D.2 | wPt3728–wPt0638 | 38.9–40 | Main effect | < 0.0001 | 25.3 |
| 2D | QYld.aww-2D.3 | wPt1301–X100006360 | 76.2–76.3 | Q * E | < 0.01 | − 335 to 62 |
| 3A | QYld.aww-3A.1 | X2254081–X1086149 | 63.3–64.3 | Q * E | < 0.001 | − 126 to 194 |
| 3A | QYld.aww-3A.2 | X1132581–X1014677 | 85.8–86.5 | Q * E | < 0.01 | 225–315 |
| 3A | QYld.aww-3A.3 | X1026080–991604 | 157.5–158.8 | Main effect | < 0.01 | − 16.1 |
| 3B | QYld.aww-3B | X989831–X1207987 | 2–2.6 | Main effect | < 0.01 | − 13.1 |
| 3DSL | QYld.aww-3DSL | 1056891–wPt6262 | 48.1–59 | Q * E | < 0.01 | 61–104 |
| 4A | QYld.aww-4A.1 | X979934–X1042486 | 53.5–56 | Main effect | < 0.0001 | 19.9 |
| 4A | QYld.aww-4A.2 | X3064552–X1125529 | 130–142.7 | Q * E | < 0.0001 | − 580 to − 34 |
| 4B | QYld.aww-4B | gwm0495–ksm0154 | 43.4–45.5 | Q * E | < 0.0001 | − 449 to 507 |
| 4D | QYld.aww-4D | X2256312–X1022538 | 8–14.7 | Q * E | < 0.01 | − 471 to 24 |
| 5A | QYld.aww-5A.1 | X1097973–X978326 | 77.7–79.6 | Q * E | < 0.01 | − 338 to 229 |
| 5A | QYld.aww-5A.2 | Vrn1A–X1264710 | 125.5–126.2 | Q * E | < 0.0001 | − 521 to − 22 |
| 5B | QYld.aww-5B.1 | X1032121–X3026027 | 115.1–115.9 | Main effect | < 0.0001 | 29.7 |
| 5B | QYld.aww-5B.2 | X3020443–X2261812 | 139.8–143.1 | Q * E | < 0.0001 | − 561 to − 36 |
| 6B | QYld.aww-6B | X996294–X1220113 | 0–1.9 | Main effect | < 0.01 | 15.2 |
| 6DLbot | QYld.aww-6DLbot | X2244522–X990024 | 14.3–17.2 | Main effect | < 0.0001 | − 17.5 |
| 6DLtop | QYld.aww-6DLtop | X3028493–X995315 | 3.7–5.2 | Main effect | < 0.01 | − 17.5 |
| 7A | QYld.aww-7A.1 | barc1055–1246868 | 45.1–56.3 | Q * E | < 0.0001 | − 517 to 1324 |
| 7A | QYld.aww-7A.2 | X1116135–9K6004 | 99.4–100.7 | Q * E | < 0.0001 | − 457 to 357 |
| 7A | QYld.aww-7A.3 | X2262955–wmc633 | 129.2–137.6 | Main effect | < 0.001 | 25.3 |
| 7A | QYld.aww-7A.4 | X2341144–X987692 | 171.5–174.5 | Q * E | < 0.0001 | − 814 |
| 7B | QYld.aww-7B | X987292–X1083752 | 6.8–29.2 | Q * E | < 0.01 | 36–163 |
| 7DS | QYld.aww-7DS.1 | WPT2551–WPT0366 | 0–1.1 | Q * E | < 0.001 | 33–68 |
| 7DS | QYld.aww-7DS.2 | WPT5049–gdm0088 | 1.8–25.4 | Main effect | < 0.01 | 21.8 |
| 7DSL | QYld.aww-7DSL | X3022993–stm0789tcacD | 13.6–21.1 | Q * E | < 0.01 | − 218 to 235 |
| Thousand grain weight | g | |||||
| 1B | QTgw.aww-1B | X1005607–X2263979 | 100–100.2 | Main effect | < 0.001 | 0.4 |
| 1D | QTgw.aww-1D.1 | wPt1799–1129001 | 88.7–102.5 | Main effect | < 0.001 | 0.4 |
| 1D | QTgw.aww-1D.2 | X988357–X1108800 | 133.9–135.3 | Q * E | < 0.001 | − 1 to 3 |
| 2A | QTgw.aww-2A.1 | X1133336–X1056356 | 24.9–30.5 | Q * E | < 0.001 | − 2 to 2 |
| 2A | QTgw.aww-2A.2 | X2277859–X2256686 | 45.9–50.1 | Q * E | < 0.001 | − 2 to − 1 |
| 2A | QTgw.aww-2A.3 | X1132500–X1094923 | 83.4–84.8 | Main effect | < 0.01 | − 0.3 |
| 2A | QTgw.aww-2A.4 | X1061775–X1038091 | 110.2–111.5 | Main effect | < 0.01 | − 0.3 |
| 2B | QTgw.aww-2B.1 | X1022997–X1082017 | 67.2–67.8 | Main effect | < 0.0001 | 0.6 |
| 2B | QTgw.aww-2B.2 | X2266150–X990132 | 78.2–78.8 | Q * E | < 0.001 | 1–2 |
| 2D | QTgw.aww-2D | X1086964–X1046316 | 69.7–70.5 | Q * E | < 0.001 | 1–4 |
| 2DS | QTgw.aww-2DS | X1162627–X1128261 | 2.6–4.3 | Main effect | < 0.0001 | − 0.4 |
| 3A | QTgw.aww-3A.1 | X1130383–wPt9562 | 96.5–97.2 | Q * E | < 0.001 | − 1 to 2 |
| 3A | QTgw.aww-3A.2 | cfa2170–X1205035 | 123.2–123.9 | Q * E | < 0.001 | − 1 to 4 |
| 3DL | QTgw.aww-3DL | X1234793–X3026282 | 1.3–1.9 | Q * E | < 0.0001 | − 5 to 1 |
| 3DSL | QTgw.aww-3DSL | X1109431–wPt2313 | 0–7.4 | Q * E | < 0.001 | − 3 to 2 |
| 4A | QTgw.aww-4A.1 | X3020781–X1072050 | 51.3–51.5 | Main effect | < 0.01 | 0.3 |
| 4A | QTgw.aww-4A.2 | X1242399–X3064552 | 124.4–130 | Q * E | < 0.0001 | − 3 to − 1 |
| 4B | QTgw.aww-4B.1 | wmc0047–wmc0349 | 22.1–23.5 | Q * E | < 0.01 | − 2 to 5 |
| 4B | QTgw.aww-4B.2 | ksm0154–DuPw0036 | 45.5–72.7 | Q * E | < 0.001 | − 2 to 6 |
| 4D | QTgw.aww-4D | X1004846–X1161775 | 35.2–37.8 | Main effect | < 0.0001 | − 0.4 |
| 5A | QTgw.aww-5A.1 | wPt9887–X1094095 | 23.3–35.8 | Q * E | < 0.01 | − 3 to 1 |
| 5A | QTgw.aww-5A.2 | wPt8226–X1070217 | 81.8–83.1 | Q * E | < 0.01 | − 2 to 1 |
| 5A | QTgw.aww-5A.3 | X1061673–X1093737 | 103.2–111 | Q * E | < 0.001 | − 2 to 1 |
| 5A | QTgw.aww-5A.4 | X1135154–Vrn1A | 123.6–125.5 | Q * E | < 0.01 | − 2 to 2 |
| 5B | QTgw.aww-5B.1 | X3023130–X1217242 | 128.2–130.2 | Q * E | < 0.01 | − 2 to − 1 |
| 5B | QTgw.aww-5B.2 | X1227599–X1240475 | 146.9–161.5 | Q * E | < 0.01 | − 2 to 1 |
| 5DSL | QTgw.aww-5DSL | cfd0019–5D–X1102031 | 94–96.2 | Q * E | < 0.01 | − 2 to 2 |
| 6A | QTgw.aww-6A.1 | X1081530–GW2 | 40.1–43.3 | Q * E | < 0.001 | 1–2 |
| 6A | QTgw.aww-6A.2 | stm0544acag–X1325639 | 58.2–66.1 | Q * E | < 0.001 | 1–2 |
| 6B | QTgw.aww-6B | X1075699–wPt5408 | 39.1–39.9 | Q * E | < 0.01 | 1–2 |
| 7A | QTgw.aww-7A.1 | 1246868–X1228158 | 56.3–56.9 | Q * E | < 0.0001 | 1–4 |
| 7A | QTgw.aww-7A.2 | X3064804–barc0070 | 85.1–85.7 | Q * E | < 0.0001 | 1–3 |
| 7A | QTgw.aww-7A.3 | 9K6680–X2262955 | 126.6–129.2 | Q * E | < 0.001 | − 1 to 1 |
| 7B | QTgw.aww-7B.1 | X987292–X1083752 | 6.8–29.2 | Q * E | < 0.01 | − 2 to 1 |
| 7B | QTgw.aww-7B.2 | X981613–X2279417 | 59.6–64.8 | Main effect | < 0.01 | − 0.3 |
| 7B | QTgw.aww-7B.2 | BSm3603_7B–wPt6156 | 109.9–110.6 | Q * E | < 0.001 | 1–3 |
| 7DS | QTgw.aww-7DS | X1103334–barc0092 | 50.2–52.3 | Main effect | < 0.01 | − 0.4 |
| 7DSL | QTgw.aww-7DSL | stm0789tcacD–wPt0789 | 21.1–29.1 | Main effect | < 0.01 | − 0.2 |
P value is derived from the Wald test for the effect indicated by Term (QTL main effect or QTL by environment interaction Q * E). QTL effect is the estimate of the effect indicated by Term, with a range of the estimates per environment that were significantly different from zero shown for Q * E effect (confidence interval of the estimate calculated as CI = estimate ± 1.96 * SE). Positive number shows allelic effect from Excalibur, negative number from Kukri