Figure 4.

Published interaction partners of the PURA protein. For all “Interactors” (left column) the method used to observe interactions is indicated by a checkmark and green background in the respective column. Well-validated interaction partners of PURA protein are marked in bold letters. For proteins, interaction partners were defined in this study as well validated when experimental evidence is available from each of the following two categories: (i) in vitro binding using recombinant proteins or in immunoprecipitation/pull-down experiments from cell lysates; (ii) colocalizing with PURA in cells or identification as PURA interactor in a high throughput proteome analysis. DNA- and RNA-interaction partners are defined as well validated if in vitro binding by PURA as well as functional validation either by a reporter assay or in an animal model has been reported. Protein interaction labeled with “1” was shown to be RNAse resistant. Functional assays labeled with “2” lacked basic statistical analyses (i.e., SD), and hence were ignored as positive score for target validation. Interaction partners labeled with “3” have only been described in a specific disease context. For target labeled with “4” ChIP-seq data have been published (Sun et al., 2020), however without providing the original data for critical assessment.