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. 2021 Mar 11;9:618335. doi: 10.3389/fcell.2021.618335

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Copyright © 2021 Saraieva, Benetos, Labat, Franco-Cereceda, Bäck and Toupance.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Telomere length dynamics in different areas of the AS and sclerotic valves. (A) Telomere length in non-calcified and calcified areas. Matched circles stand for individual patients. Statistical analysis was performed using paired t-test. (B) Representative picture of telomerase activity evaluation by TRAP in non-calcified and calcified areas of the valve tissue (n = 4 for each type of tissue). Lines correspond to telomerase activity in 1 μg of protein extract. Samples, which contain HeLa cell extract mixed with tissue extract, represent controls for false-negative results and heat-treated samples serve as controls for false-positive results. Extracts from HeLa cells were used as the positive control. kb, kilobase.