TABLE 2.
Expression levels (fold change) of RND-family (AdeB, AdeJ, AdeG) and MATE-family efflux pumps (AbeM) detected by reverse transcriptase quantitative PCR (RT-qPCR) in Acinetobacter baumannii that showed reduction of MIC for fluoroquinolones in presence of efflux pump inhibitors.
| Strain No | MLST/Clonal complex | adeB fold change | adeJ fold change | adeG fold change | abeM fold change |
| A_112 | ST-32/CC32 | 3.6 | 2.1 | 1.2 | 0.2 |
| A_113 | ST032/CC32 | 3.7 | 4.1 | 1.1 | 2 |
| A_117 | ST-10/CC10 | ND | 2.1 | 5.6 | ND |
| A_124 | ST-1440/CC218 | 3.03 | ND | ND | 0.29 |
| A_125 | ST-2/CC2 | 7.0 | 2.8 | 7.7 | 2.69 |
| A_130 | ST-575/CC10 | 0.06 | 2.9 | 0.14 | 0.6 |
| A_131 | ST-902/CC1 | 34 | 2 | 0.73 | 1.4 |
| A_132 | ST-10/CC10 | 0.044 | 3.5 | 1.3 | 4.47 |
| A_136 | ST-149/CC149 | 5 | ND | ND | 0.7 |
| A_138 | ST-905/CC32 | 8.65 | 3.1 | 0.8 | 2.0 |
| A_145 | ST-149/CC149 | 12.7 | 0.7 | 0.6 | 0.8 |
| A_146 | ST-1406/CC10 | 3 | 0.1 | ND | 0.1 |
| A_149 | ST-149/CC149 | 2.2 | 0.2 | 0.7 | 0.4 |
| A_150 | ST-10/CC10 | ND | 3.79 | ND | 0.49 |
| A_152 | ST-623CC1 | 1.5 | 1.2 | 0.4 | 5.9 |
| A_153 | ST-623/CC1 | 1.1 | 0.5 | 13.9 | 7.5 |
| A_155 | ST-10/CC10 | 5.4 | 3.1 | 0.3 | 0.5 |
| A_158 | ST-1483/CC10 | 22 | 1.5 | 2.5 | 1.1 |
| A_159 | ST-1483/CC10 | ND | 2.4 | ND | 1.2 |
| A_160 | ST-1/CC1 | 0.1 | ND | ND | 0.12 |
| A_161 | ST-149/CC149 | 21.5 | 0.1 | 0.8 | 0.9 |
| A_162 | ST-1406/CC10 | 17.5 | 2.8 | 0.03 | 0.2 |
| A_163 | ST-575/CC10 | 20 | 2.8 | 0.007 | 0.6 |
| A_166 | ST-625/CC513 | 11.0 | 10.7 | 3.2 | 1.05 |
| A_167 | ST-2/CC2 | 25.3 | 2.5 | 2.8 | 1.7 |
| A_172 | ST-526/CC2 | 9.8 | 0.5 | 1.8 | 4 |
| A_173 | ST-526/CC2 | 18.35 | 10.64 | 17.4 | 1.097 |
| A_177 | ST-1484 (singleton) | 0.021 | 0.757 | 0.383 | 25 |
| A-179 | ST-7/CC1 | 15.0 | 0.6 | 0.25 | 1.1 |
RND, Resistance Nodulation Division family; MATE, Multidrug and Toxin Extrusion family; MIC, Minimum Inhibitory Concentration; ND, No Detection. The relative gene expression (△CT) for pump genes transcripts was calculated against that for the 16S rRNA gene, [△CT of test gene= CT of test gene − CT of 16S] and the △△CT was calculated against that for the ciprofloxacin-susceptible strain, A. baumannii ATCC 19606 (expression = 1), which served as the control. Relative expression levels of pump genes were calculated by the 2−ΔΔCT method. Expression level of pump gene which was ≥2-fold higher in comparison to the control strain A. baumannii ATCC 19606 (expression = 1), indicated as bold and considered as overexpression of the pump.