Fig. 5.

Intracellular binding of anti-ALFA_Nb_sfGFP (ALFA tag system). Confocal images of HeLa cells 24 h after transient transfection with constructs indicated at the left of each row and imaged for the channels indicated at the top of each row. The first column represents the GFP channel (green), the second column is the mCherry channel (red), the third column is the overlay of the two channels, showing the colocalization (indicated in yellow) of the anti-ALFA_Nb_sfGFP with the respective mitochondrial (B), nuclear (C) and filament (D) baits; the fourth column represents the nuclear Hoechst staining (blue) and the fifth column is the merge of all three channels. Scale bars: 15 µm. The figures are from a representative experiment, performed at least three times.