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. 2021 Mar 25;188(4):137. doi: 10.1007/s00604-021-04766-5

Scheme 1.

Scheme 1

Schemtaic for the FRET assay used for screening the peptides library and the assay development. (A) Fluorescent molecule and quencher attached to the peptide seqeunce (low fluorescence signal); (B) protease cleaves the peptide seqeunce and separate the fluorecent molecules from the quencher, which results in increase in the fluorescence signal