Figure 7.

Treatment with recombinant IL-19 did not alter macrophage phenotype. (A) Representative immunohistological images of L5 lumbar spinal cord sections from EAE mice. Pro-inflammatory M1 macrophage (iNOS) were examined. (B) Quantitative data of A. IL-19 treatment did not alter the number of iNOS⁺ F4/80⁺ M1 cells albeit F4/80⁺ cells were not detected in IL-19–treated WT EAE mice. (C) Representative immunohistological images of L5 lumbar spinal cord sections from EAE mice. Anti-inflammatory M2 macrophage (arginase 1) were examined. (D) Quantitative data of (C). IL-19 treatment did not alter M1/M2 cell population although F4/80⁺ cells were not detected in IL-19–treated WT EAE mice. Scale bars, 100 μm. Statistical significance was analyzed using repeated measures ANOVA followed by post-hoc Tukey's test. Data represent means ± SEM (n = 5). ND, not detected.