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. 2021 Mar 24;12(4):314. doi: 10.1038/s41419-021-03605-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Tumor primary cells were collected from the tumor-bearing mice and detected by flow cytometry. A TIL and tumor infiltrating DCs were analyzed by staining with CD45 and CD11c. The results were represented as percentage in tumors. B Tumor infiltrating T cells were analyzed by staining with CD3, CD4, and CD8. The absolute numbers of CD4⁺ and CD8⁺ T cells in tumors were expressed as mean ± SD. C Mouse tumor samples were stained using IHC for CD80, CD86, and CD69 expressions. Cell numbers were counted under a 40× objective. Each dot denotes the immune cell number from a tumor section. Mean and standard deviation of immune cell number in each group are shown. Scale bar = 40 μm. n = 5, **p < 0.01 vs. control. CON, control.