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Activities of WT and C446 mutated SaAMs.
| Enzyme | Specific activities (U/mg protein) | ||||
|---|---|---|---|---|---|
| Starch tranglycosylation | Disproportionation | Cyclization | Coupling | Hydrolysis | |
| WT | 57 ± 2 | 54 ± 1 | 0.9 ± 0.2 | 0.19 ± 0.01 | 0.05 ± 0.01 |
| C446A | 73 ± 1 | 70 ± 1 | 1.3 ± 0.2 | 0.16 ± 0.02 | 0.03 ± 0.01 |
| C446P | 46 ± 1 | 51 ± 1 | 0.7 ± 0.1 | 0.12 ± 0.01 | 0.02 ± 0.01 |
| C446S | 56 ± 1 | 60 ± 1 | 0.8 ± 0.1 | 0.15 ± 0.01 | 0.04 0.01 |
Data are shown as the mean ± standard deviation and are derived from three independent experiments.
