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. 2021 Mar 24;11:6775. doi: 10.1038/s41598-021-86028-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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qRT-PCR and immunocytochemical analyses for PDAC cells in 2D culture. (a) E-cadherin and (b) vimentin mRNA levels were examined by qRT-PCR. There was high variability in the E-cadherin and vimentin levels. Results are presented as the means ± SD from triplicate measurements. The results are shown after normalization against the values obtained for MIA PaCa-2 cells (value = 1). (c) The expression levels of E-cadherin and vimentin were determined by immunocytochemical analysis of the eight human PDAC cell lines and the HPDE6 cells. Scale bar = 20 μm.