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. 2021 Mar 24;11:6738. doi: 10.1038/s41598-021-86228-9

Table 3.

The melting temperature values (Tm) of four barcode regions derived from Mitragyna species.

Plant name Melting temperature (Tm) (°C)
ITS2 matK rbcL trnH-psbA intergenic spacer
M. speciosa 83.9 ± 0.06 75.0 ± 0.06 79.0 ± 0.06 74.5 ± 0.06
M. diversifolia 85.2 ± 0.06 74.6 ± 0.00 78.2 ± 0.06 74.8 ± 0.06
M. hirsuta 85.2 ± 0.06 74.6 ± 0.00 78.2 ± 0.06 74.8 ± 0.06
M. rotundifolia 85.2 ± 0.06 74.6 ± 0.00 78.2 ± 0.06 74.8 ± 0.00

SD-standard deviation.