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. 2021 Mar 24;11:6769. doi: 10.1038/s41598-021-85366-4

Figure 1.

Figure 1

pHcoCas9-induced expression upon supplying the RNA silencing suppressor P19. (a) The binary vector construct of pHcoCas9 used to deliver the Cas9 endonuclease and the P19 construct used to deliver P19 suppressor protein. (b) pHcoCas9-delivered Cas9 protein expression co-infiltrated with and without a plasmid expressing P19 in N. benthamiana from 3 to 9 days post infiltration (dpi). The top panels for each day are western blots used to detect Cas9 protein (164-kDa). The bottom panels are protein gels stained with Coomassie using Rubisco (55-kDa) as a loading control.