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. 2021 Mar 24;11:6769. doi: 10.1038/s41598-021-85366-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Indel analysis of Cas9/sgRNA using P19, a viral RNA silencing suppressor. (a) The TRBO-based construct used to deliver the gGFP (TRBO-G-3′gGFP). The pHcoCas9 construct of Fig. 1 is also shown here for convenience of reference. (b) Indel analysis time course experiment using a BsgI restriction digest assay of 16c transgenic plants co-infiltrated with pHcoCas9 and TRBO-G-3′gGFP, with and without P19. Treated tissues were sampled at 2, 4, 6, 8, and 10 dpi. The red arrow indicates indel-containing DNA.