Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 24;11:6769. doi: 10.1038/s41598-021-85366-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

P19 effect on indel formation in Cas9/sgRNA infiltrated plants of different ages. (a) Photos of 16c plants infiltrated with TRBO-HcoCas9 and TRBO-G-3′gGFP at the age of 3 weeks (3-wk) or 5 weeks (5-wk) taken at 10 dpi. (b,d) BsgI restriction enzyme resistance assays of 3-wk-old and 5-wk-old 16c plants, respectively, co-infiltrated with either TRBO-HcoCas9 or pHcoCas9 expression constructs both with and without P19 and/or TRBO-G-3′gGFP. At 10 dpi samples were

taken from 16c plants that were treated either at the age of 3-wk or 5-wk. The red arrow indicates indel-containing DNA. The average and standard deviation (varying between ± 2.2 and ± 8) of indel percentages of 3-wk treated plants were calculated based on three independent biological replicates. However, for the 5-wk plants the compilations were more complicated and such analyses were not performed so they are only indicative of a trend and not of absolute differences. (c,e) Western blot showing Cas9 protein accumulation (164-kDa) corresponding to P19 protein expression (19-kDa) at 7 dpi in 3-wk and 5-wk-old infiltrated 16c plants.