Glutamine depletion changes CD24 surface expression (a) and (b). Flow cytometry data showing effect of gln-deprived media and DON containing complete media on CD24 expressing population in MCF-7 after 72 and 8 days of treatment. (c) and (d) Flow cytometry data showing effect of gln-deprived media and DON containing complete media on CD24 expressing population in MDAMB-231 cells after 72 and 8 days of treatment. Data was analyzed using two-way ANOVA, each bar represents n = 3, ± SD, *p < 0.05, **p < 0.01, ***p < 0.001