Fig. 6.

qPCR analysis of stem cell markers: (a) and (b) mRNA expression of stem cell markers Sox-2, oct-4 and Nanog in MCF-7 and MCF-7-bCSCs after growing cells in gln-deprived media and DON-containing complete media for 72 h. (c) and (d) mRNA expression of stem cell markers Sox-2, oct-4 and Nanog in MDAMB-231 and MDAMB-231-bCSCs after growing cells in gln-deprived media and DON containing complete media for 72 h. Statistical analysis: one-way-ANOVA, each bar represents n = 3, ± SD, *p < 0.05, **p < 0.01, ***p < 0.001