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. 2021 Mar 11;12:637982. doi: 10.3389/fimmu.2021.637982

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Copyright © 2021 Srivastava, Niu, Phadke, Bellaire and Cho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Antibody responses against the RBD immunogen. (A) Immunization and blood collection schedule. (B) ELISA of pooled antisera collected about 2 weeks after each immunization. ELISA was also done using HIV-1 gp41-28×3 to assess antibodies elicited against the 6×His tag (right panel). (C) ELISA of antisera from individual animals after the third immunization.