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. 2021 Mar 11;12:637982. doi: 10.3389/fimmu.2021.637982

Figure 4.

Figure 4

Detailed analyses of neutralizing antibody responses. (A) RT-qPCR analyses of virus neutralization assay. Cell culture supernatants from neutralization assays using antisera after the 2nd immunization were evaluated in triplicate. Viral RNA was harvested from culture media of virus-infected cells. Following reverse transcription, qPCR was carried out using IDT 2019-nCoV RUO Kit. (B) Extended nAb titration of antiserum from individual mouse in the Alhydrogel group after 3rd immunizations. Pooled sera were also analyzed for comparison.