Figure 8.

Western blotting of protein samples extracted from post-ischemic kidneys on day 2 after IRI. (A) Overall expression of TLR2 and TLR4 tended to be higher in post-ischemic kidneys of mice fed an HF or HS diet for 1 week compared to those of the mice fed a normal diet. (B,C) The expression of TLR-2 and TLR-4 was significantly higher in the HF, HS, and HF+HS diet groups fed for 6 weeks compared to the control group. *P < 0.05, compared with the control group (n = 3–4 for each group). Statistical analysis was performed using the Mann-Whitney U-test. HF, high-fat; HS, high-salt; HF+HS, high-fat with high-salt.