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. 2021 Mar 11;9:649288. doi: 10.3389/fbioe.2021.649288

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Copyright © 2021 Citeroni, Mauro, Ciardulli, Di Mattia, El Khatib, Russo, Turriani, Santer, Della Porta, Maffulli, Forsyth and Barboni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental design. (A) Analysis of conditioned media (CM) derived from FT alone (CM_FT) and from co-culture system (CM_CO) on AECs cultured for 14 days prior to 1:1 dilution with standard media (SM) in air (21% O₂) compared with AECs cultured alone (CTR) and in co-culture system (CO: FT+AECs). (B) Analysis of fresh collected CM after storage at −80°C (CM_FZ) on AECs cultured in either air or and physoxia (2% O₂) prior to 1:1 dilution with SM. FT, fetal tendons explants; AECs, amniotic epithelial stem cells. CO, co-culture with AECs and FT; CM_FT, conditioned media of FT; CM_CO, conditioned media of CO; CM_FZ, CM after freezing thawing procedure; 21% O₂, air oxygen incubator; 2% O₂, incubator at 2%; SM, standard media.