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. 2021 Mar 11;9:649288. doi: 10.3389/fbioe.2021.649288

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Copyright © 2021 Citeroni, Mauro, Ciardulli, Di Mattia, El Khatib, Russo, Turriani, Santer, Della Porta, Maffulli, Forsyth and Barboni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of tenogenic potential of CM_COFZ 2%. Real time qPCR analysis for (A) EMT genes and (B) tendon related genes on samples in AECs cells cultured for 14 days in different experimental conditions. Relative quantification of each mRNA gene expression normalized to endogenous GAPDH (internal control) was calculated using the ΔΔCt method and presented as fold change over the AECs T0 =1 (calibrator; dashed line). (C) Representative Western Blot image for α-TNMD and α-TUBULIN proteins expression. (D) α-TNMD protein quantification normalized on α-TUBULIN expression levels and expressed as means ± S.D of three replicates/each experimental condition. Values were considered significant for p < 0.05, with the indicated superscripts ^*vs. CTR, ^#vs. CM_COFZ, ^§vs. CTR 2%,^£vs. T0.