Table 1.
Primers details used for RT-qPCR analysis.
| Gene | Accession number | Sequences | Product size (bp) |
|---|---|---|---|
| VIMa | XM_004014247.4 | For: 5′-GACCAGCTCACCAACGACA-3′ | 93 |
| Rev: 5′-CTCCTCCTGCAACTTCTCCC-3′ | |||
| SNAILa | XM_004014881.2 | For:5′- GTCGTGGGTGGAGAGCTTTG−3′ | 119 |
| Rev: 5′- TGCTGGAAAGTGAGCTCTGG−3′ | |||
| TWISTa | XM_004008211.4 | For: 5′-GCCGGAGACCTAGATGTCATTG-3′ | 150 |
| Rev: 5′-CCACGCCCTGTTTCTTTGAAT-3′ | |||
| TNMDb | NM_001099948.1 | For: 5′-TGGTGAAGACCTTCACTTTCC-3′ | 352 |
| Rev: 5′-TTAAACCCTCCCCAGCATGC-3′ | |||
| SCXBb | XM_866422.2 | For: 5′-AACAGCGTGAACACGGCTTTC-3′ | 299 |
| Rev: 5′-TTTCTCTGGTTGCTGAGGCAG-3′ | |||
| COL Ib | AF129287.1 | For: 5′-CGTGATCTGCGACGAACTTAA-3′ | 212 |
| Rev: 5′-GTCCAGGAAGTCCAGGTTGT-3′ | |||
| COL IIIb | AY091605.1 | For: 5′-AAGGGCAGGGAACAACTTGAT-3′ | 355 |
| Rev: 5′-GTGGGCAAACTGCACAACATT | |||
| THSB4b | NM_001034728.1 | For: 5′-CCGCAGGTCTTTGACCTTCT-3′ | 231 |
| Rev: 5′-CAGGTAACGGAGGATGGCTTT-3′ | |||
| HIF 1α | XM_027971913.1 | For: 5′-TGCTCATCAGTTGCCACTTC-3′ | 311 |
| Rev: 5′-TTTCCTCATGGTCACATGGAT-3′ | |||
| GAPDHb | AF030943.1 | For: 5′-CCTGCACCACCAACTGCTTG-3′ | 224 |
| Rev: 5′-TTGAGCTCAGGGATGACCTTG-3′ |