FIGURE 2.

Schematic (A) and photo (B) of the ex vivo preparation for functional recordings from afferents innervating distal colon and rectum (colorectum). The colorectum, spinal nerves, and T12—S1 DRGs were harvested from mice in continuity. The colorectum was cannulated and stimulated mechanically by graded pressure distension and mucosal shearing. The photo in (B) was from stitching multiple photos taken under a stereomicroscope. LSN, lumbar splanchnic nerve; PN, pelvic nerve; IMG, inferior mesenteric ganglion; MPG, major pelvic ganglion; CG, celiac ganglion.