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. 2021 Mar 11;9:640450. doi: 10.3389/fbioe.2021.640450

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Copyright © 2021 Grosjean, Guénard, Giraud, Muller, Plésiat and Juarez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic representation of targeted genomic regions for gene inactivation in strain SM54. Approximative position of the 37 genes deleted from the wild-type strain PAO1 in the physical map of its chromosome (Accession No. NC_002516). Genes related to antibiotic resistance are marked in blue, those related to virulence factors are marked in red, those related to cytotoxicity are marked in green and finally those genes related to adhesion are marked in yellow.