TABLE 1.
Strains and plasmids used in this study.
| Strains | Relevant characteristics | Sources or references |
| Pseudomonas aeruginosa | ||
| PAO1 | Wild-type reference strain, prototroph | Stover et al., 2000 |
| SMEff | PAO1ΔmexAB,mexCD,mexEF,mexXY | This study |
| SMRes | SMEffΔampC,aph,arnBCADTEFugd | This study |
| SMVir | PAO1ΔexoS,exoT,exoY,fliEFG,lasA,pilQ,plcH,pqsA,rhlA,toxA | This study |
| SM54 | SMRes-VirΔlecA,lecB,pcrVHpopBD | This study |
| Escherichia coli | ||
| DH5α | F– ϕ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rk–, mk+) phoA supE44 thi-1 gyrA96 relA1 λ– | Invitrogen |
| CC118 | Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE (Am) recA1 | Manoil and Beckwith, 1985 |
| CC118λpir | Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE (Am) recA1, lysogenic for phage λpir | Herrero et al., 1990 |
| HB101 | supE44 hsdS20(rB– mB–) recA13 ara-14 pro A2 lacY1 galK2 rpsL20 xyl-5 mtl-1 leuμB6 thi-1 | Lacks and Greenberg, 1977 |
| Plasmids | ||
| pRK2013 | Helper plasmid for mobilization of non-self-transmissible plasmids, mob1, tra1 col E1, KmR | Kaniga et al., 1991 |
| pKNG101 | Suicide vector oriR6K sacB insB, SmR | Kaniga et al., 1991 |
KmR: kanamycin resistance; SmR: streptomycin resistance.